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Soft Agar Gelatin Overlay

[所属分类:培养基配方] [发布时间:2021-12-16] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per plate:

Base agar....................................................... 15.0mL

Soft agar gelatin overlay................................. 2.5mL

pH 7.0 ± 0.2 at 25°C

Base Agar:

Composition per liter:

Agar ..................................................................15.0g

Peptone................................................................5.0g

NaCl....................................................................5.0g

Beef extract.........................................................3.0g

MnSO4·H2O ......................................................0.05g

Preparation of Base Agar: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 45°–50°C.

Soft Agar Gelatin Overlay:

Composition per liter:

Gelatin...............................................................15.0g

Agar ....................................................................8.0g

Peptone................................................................5.0g

NaCl....................................................................5.0g

Beef extract.........................................................3.0g

MnSO4·H2O ......................................................0.05g

Preparation of Soft Agar Gelatin Overlay:

Add components to distilled/deionized water and

bring volume to 1.0L. Mix thoroughly. Gently heat

and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C.

Preparation of Medium: Aseptically pour

cooled, sterile base agar into sterile Petri dishes in

15.0mL volumes. Allow agar to solidify. Inoculate

plates with samples. Overlay each plate with 2.5mL

of soft agar gelatin overlay.

Use: For the cultivation and differentiation of micro

organisms based on proteolytic activity.