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Sorbitol Agar

[所属分类:培养基配方] [发布时间:2021-12-16] [发布人:网站管理员2] [阅读次数:] [返回]

Sorbitol Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................20.0g

Peptone..............................................................10.0g

NaCl....................................................................2.0g

Yeast extract........................................................2.0g

Sorbitol solution............................................ 50.0mL

pH 7.0 ± 0.2 at 25°C

Sorbitol Solution:

Composition per 50.0mL:

Sorbitol................................................................5.0g

Preparation of Sorbitol Solution: Add sorbitol

to distilled/deionized water and bring volume to

50.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept sorbitol solution, to distilled/deionized water

and bring volume to 950.0mL. Mix thoroughly. Ad

just pH to 7.0. Gently heat and bring to boiling. Au

toclave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C. Aseptically add sterile sorbitol solution.

Mix thoroughly. Pour into sterile Petri dishes or dis

tribute into sterile tubes.

Use: For the cultivation and maintenance of

Pseudomonas species.