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Sorbitol MacConkey Agar (MacConkey Agar with Sorbitol)

[所属分类:培养基配方] [发布时间:2021-12-16] [发布人:网站管理员2] [阅读次数:] [返回]

Sorbitol MacConkey Agar

(MacConkey Agar with Sorbitol)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone .............................................................20.0g

Agar ..................................................................15.0g

Sorbitol .............................................................10.0g

NaCl....................................................................5.0g

Bile salts No. 3....................................................1.5g

Neutral Red.......................................................0.03g

Crystal Violet...................................................1.0mg

pH 7.1 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems and Oxoid.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Pour into sterile Petri dish

es or leave in tubes.

Use: For the isolation and cultivation of pathogenic

Escherichia coli.