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ST Holding Medium (m-ST Holding Medium)

[所属分类:培养基配方] [发布时间:2021-12-17] [发布人:网站管理员2] [阅读次数:] [返回]

ST Holding Medium

(m-ST Holding Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

KH2PO4...............................................................3.0g

Tris(hydroxymethyl)aminomethane buffer.........3.0g

Sulfanilamide......................................................1.5g

NaH2PO4·H2O.....................................................0.1g

Ethanol (95% solution) ................................. 10.0mL

pH 8.6 ± 0.2 at 25°C

Preparation of Medium: Dissolve the sulfanil

amide in the ethanol. Add all components to distilled/

deionized water and bring volume to 1.0L. Mix thor

oughly. Autoclave for 15 min at 15 psi pressure–

121°C. Distribute in 1.8mL volumes to sterile Petri

dishes with tight-fitting lids and an absorbent filter.

Use: For the cultivation and enumeration of coliform

bacteria by the delayed-incubation total coliform

procedure. For use as a holding or transport medium

to keep coliform bacteria viable between sampling

and laboratory culture.