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STAA Agar Base

[所属分类:培养基配方] [发布时间:2021-12-17] [发布人:网站管理员2] [阅读次数:] [返回]

STAA Agar Base

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone .............................................................20.0g

Agar ..................................................................13.0g

Glycerol ..............................................................7.5g

Yeast extract........................................................2.0g

K2HPO4...............................................................1.0g

MgSO4·7H2O......................................................1.0g

STAA selective supplement.......................... 10.0mL

pH 7.0 ± 0.1 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

STAA Selective Supplement:

Composition per 10.0mL:

Streptomycin sulfate ...........................................0.5g

Cycloheximide..................................................0.05g

Thallous acetate ................................................0.05g

Preparation of STAA Selective Supplement:

Add components to distilled/deionized water and

bring volume to 10.0mL. Mix thoroughly. Filter ster

ilize.

Preparation of Medium: Add components, ex

cept glycerol and STAA selective supplement, to dis

tilled/deionized water and bring volume to 985.0mL.

Mix thoroughly. Gently heat and bring to boiling.

Add glycerol. Mix thoroughly. Autoclave for 15 min

at 15 psi pressure–121°C. Cool to 45°–50°C. Asepti

cally add sterile STAA selective supplement. Mix

thoroughly. Pour into sterile Petri dishes or distribute

into sterile tubes.

Use: For the isolation of Brochothrix thermosphacta

from meat products.