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Staphylococcus/Streptococcus Selective Medium
[所属分类:培养基配方] [发布时间:2021-12-17] [发布人:网站管理员2] [阅读次数:] [返回]
Staphylococcus/Streptococcus
Selective Medium
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per 1060.0mL:
Columbia blood agar base ................................. 1.0L
Horse blood, defibrinated ............................. 50.0mL
Antibiotic inhibitor ....................................... 10.0mL
pH 7.3 ± 0.2 at 25°C
Columbia Blood Agar Base:
Composition per liter:
Special peptone.................................................23.0g
Agar ..................................................................10.0g
NaCl....................................................................5.0g
Starch ..................................................................1.0g
Source: Columbia blood agar base is available as a
premixed powder from Oxoid.
Preparation of Columbia Blood Agar Base:
Add components to distilled/deionized water and
bring volume to 1.0L. Mix thoroughly. Gently heat
and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C.
Antibiotic Inhibitor:
Composition per 10.0mL:
Nalidixic acid..................................................0.015g
Colistin sulfate ..................................................0.01g
Ethanol (95% solution) ................................. 10.0mL
Preparation of Antibiotic Inhibitor: Add com
ponents to 10.0mL ethanol. Mix thoroughly. Filter
sterilize.
Preparation of Medium: To 1.0L of cooled ster
ile Columbia blood agar base, aseptically add sterile
horse blood and sterile antibiotic inhibitor. Mix thor
oughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use: For the selective isolation of Staphylococcus
aureus and streptococci from Starch Agar
Composition per liter:
Agar ..................................................................12.0g
Soluble starch....................................................10.0g
Beef extract.........................................................3.0g
pH 7.5 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to 1.0L.
Mix thoroughly. Gently heat and bring to boiling.
Distribute into tubes or flasks. Autoclave for 15 min
at 15 psi pressure–121°C. Pour into sterile Petri dish
es.
Use: For the cultivation and differentiation of a vari
ety of microorganisms based on amylase production.
After incubation, starch hydrolysis is determined by
the addition of Gram’s or Lugol’s iodine solution. Or
ganisms that produce amylase appear as colonies sur
rounded by a clear zone.