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Starch Agar

[所属分类:培养基配方] [发布时间:2021-12-17] [发布人:网站管理员2] [阅读次数:] [返回]

Starch Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................12.0g

Soluble starch....................................................10.0g

Beef extract.........................................................3.0g

pH 7.5 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Distrib

ute into tubes or flasks. Autoclave for 15 min at 15 psi

pressure–121°C. Pour into sterile Petri dishes.

Use: For the cultivation and differentiation of a vari

ety of microorganisms based on amylase production.

After incubation, starch hydrolysis is determined by

the addition of Gram’s or Lugol’s iodine solution. Or

ganisms that produce amylase appear as colonies sur

rounded by a clear zone.