培养基配方

您现在的位置：网站首页 >> 培养基配方

Starch Hydrolysis Agar

[所属分类:培养基配方] [发布时间:2021-12-17] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Beef heart, infusion from................................500.0g

Soluble starch....................................................20.0g

Agar ..................................................................15.0g

Tryptose ............................................................10.0g

NaCl....................................................................5.0g

pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Pour into sterile Petri dish

es or leave in tubes.

Use: For the cultivation and differentiation of a vari

ety of microorganisms based on amylase production.

After incubation, starch hydrolysis is determined by

the addition of Gram’s or Lugol’s iodine solution. Or

ganisms that produce amylase appear as colonies sur

rounded by a clear zone.