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Sterility Test Broth (USP Alternative Thioglycolate Medium)

[所属分类:培养基配方] [发布时间:2021-12-17] [发布人:网站管理员2] [阅读次数:] [返回]

Sterility Test Broth

(USP Alternative

Thioglycolate Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein................................15.0g

Glucose ...............................................................5.0g

Yeast extract........................................................5.0g

NaCl....................................................................2.5g

L-Cystine .............................................................0.5g

Sodium thioglycolate ..........................................0.5g

pH 7.1 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Cool to 25°C. If not used

immediately, prior to inoculation heat tubes in a boil

ing water bath for 5–10 min. Cool to 25°C.

Use: As an alternate medium, instead of fluid

thioglycolate broth, for testing the sterility of a vari

ety of specimens.