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培养基配方

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Succinate Mineral Medium

[所属分类:培养基配方] [发布时间:2021-12-17] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1001.0mL:

MnSO4·7H2O ....................................................62.0g

K2HPO4·3H2O...................................................1.63g

NH4Cl ...............................................................1.02g

KH2PO4.............................................................0.39g

Succinate...................................................... 10.0mM

Trace elements solution .................................. 1.0mL

pH 6.8 ± 0.2 at 25°C

Trace Elements Solution:

Composition per 100.0mL:

Solution A..................................................... 50.0mL

Solution B ..................................................... 50.0mL

Preparation of Trace Elements Solution:

Aseptically combine 50.0mL of sterile solution A

with 50.0mL of sterile solution B.

Solution A:

Composition per liter:

Disodium EDTA ...............................................0.25g

CaCl2·2H2O.......................................................0.14g

FeCl2·4H2O .......................................................0.14g

Preparation of Solution A: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Autoclave for 15 min at 15 psi pres

sure–121°C.

Solution B:

Composition per liter:

Disodium EDTA ...............................................0.25g

H3BO3 ............................................................30.0mg

CoCl2·2H2O ...................................................28.0mg

ZnCl2................................................................5.0mg

MnCl2·4H2O ....................................................4.0mg

NaMoO4·2H2O.................................................3.0mg

NiCl2·6H2O....................................................1.65mg

CuCl2·2H2O .....................................................0.7mg

Preparation of Solution B: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Autoclave for 15 min at 15 psi pres

sure–121°C.

Preparation of Medium: Add components, ex

cept trace elements solution, to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Au

toclave for 15 min at 15 psi pressure–121°C. Asepti

cally add 1.0mL of sterile trace elements solution.

Mix thoroughly. Aseptically distribute into sterile

tubes or flasks.

Use: For the cultivation of bacteria that can utilize

succinate as a carbon source.

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Succinate Mineral Medium