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Sucrose Agar

[所属分类:培养基配方] [发布时间:2021-12-17] [发布人:网站管理员2] [阅读次数:] [返回]

Sucrose Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sucrose..............................................................50.0g

Agar ..................................................................20.0g

Peptone .............................................................10.0g

NaCl....................................................................5.0g

Yeast extract........................................................5.0g

CaCO3 .................................................................3.0g

Phenylethyl alcohol ............................................3.0g

MgSO4·7H2O......................................................0.5g

MnSO4·4H2O......................................................0.5g

Tween 80.............................................................0.1g

Bromcresol Green..........................................20.0mg

Cycloheximide solution................................ 10.0mL

pH 6.2 ± 0.2 at 25°C

Cycloheximide Solution:

Composition per 10.0mL:

Cycloheximide..................................................0.01g

Preparation of Cycloheximide Solution: Add

cycloheximide to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add agar to distilled/

deionized water and bring volume to 500.0mL. Mix

thoroughly. Gently heat and bring to boiling. Add re

maining components, except cycloheximide solution

and phenylethyl alcohol, and bring volume to

990.0mL with distilled/deionized water. Adjust pH to

6.2. Autoclave for 15 min at 15 psi pressure–121°C.

Cool to 45°–50°C. Aseptically add 10.0mL of sterile

cycloheximide solution and 3.0g of phenylethyl alco

hol. Mix thoroughly. Pour into sterile Petri dishes or

distribute into sterile tubes.

Use: For the isolation and cultivation of Lactobacil

lus species from brewery isolates.