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T7 Agar Base (m-T7 Agar Base)

[所属分类:培养基配方] [发布时间:2021-12-20] [发布人:网站管理员2] [阅读次数:] [返回]

T7 Agar Base

(m-T7 Agar Base)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Lactose..............................................................20.0g

Agar ..................................................................15.0g

Polyoxyethylene ether W-1.................................5.0g

Yeast extract........................................................3.0g

Pancreatic digest of casein..................................2.5g

Peptic digest of animal tissue..............................2.5g

Sodium heptadecyl sulfate..................................0.1g

Bromthymol Blue ...............................................0.1g

Bromcresol Purple ..............................................0.1g

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat while stirring and bring

to boiling. Distribute into tubes or flasks. Autoclave

for 15 min at 15 psi pressure–121°C. Cool to 45°–

50°C. The medium may be made more selective by

adding 1.0mg of penicillin G per liter. Pour into ster

ile Petri dishes or leave in tubes.

Use: For the selective recovery and differential iden

tification of injured coliform microorganisms from

chlorinated water by the membrane filter method.

For rapid estimation of the bacteriological quality of

water using the membrane filter method.