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TCH Medium (Thiophene 2 Carboxylic Acid Hydrazide Medium)

[所属分类:培养基配方] [发布时间:2021-12-20] [发布人:网站管理员2] [阅读次数:] [返回]

TCH Medium

(Thiophene 2 Carboxylic

Acid Hydrazide Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1105.0mL:

Thiophene-2-carboxylic acid hydrazide ..........1.1mg

Middlebrook 7H10 agar base............................. 1.0L

OADC enrichment...................................... 100.0mL

Glycerol .......................................................... 5.0mL

pH 6.6 ± 0.2 at 25°C

Middlebrook 7H10 Agar Base:

Composition per liter:

Agar ..................................................................15.0g

Na2HPO4.............................................................1.5g

KH2PO4...............................................................1.5g

(NH4)2SO4 ..........................................................0.5g

L-Glutamic acid...................................................0.5g

Sodium citrate.....................................................0.4g

Ferric ammonium citrate...................................0.04g

MgSO4·7H2O..................................................0.025g

ZnSO4·7H2O ....................................................1.0mg

CuSO4·5H2O....................................................1.0mg

Pyridoxine........................................................1.0mg

Biotin ...............................................................0.5mg

CaCl2·2H2O......................................................0.5mg

Malachite Green.............................................0.25mg

Preparation of Middlebrook 7H10 Agar

Base: Add glycerol to 900.0mL of distilled/deion

ized water and add remaining components. Mix thor

oughly. Gently heat and bring to boiling.

Middlebrook OADC Enrichment:

Composition per 100.0mL:

Bovine albumin fraction V .................................5.0g

Glucose ...............................................................2.0g

NaCl..................................................................0.85g

Oleic acid ..........................................................0.05g

Catalase............................................................4.0mg

Source: This enrichment is available as a prepared

enrichment from BD Diagnostic Systems.

Preparation of Middlebrook OADC Enrich

ment: Add components to distilled/deionized water

and bring volume to 100.0mL. Mix thoroughly. Filter

sterilize.

Preparation for Medium: Combine compo

nents. Mix thoroughly. Distribute into tubes or flasks.

Autoclave for 15 min at 15 psi pressure–121°C. Pour

into sterile Petri dishes or leave in tubes.

Use: For the differentiation of Mycobacterium spe

cies based on sensitivity to TCH. Mycobacterium bo

vis is inhibited by TCH. Mycobacterium tuberculosis

and other mycobacteria are generally resistant to low

concentrations of TCH. This distinguishes Mycobac

terium bovis from other nonchromogenic, slow

growing mycobacteria.

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TCH Medium (Thiophene 2 Carboxylic Acid Hydrazide Medium)