培养基配方

您现在的位置：网站首页 >> 培养基配方

Tergitol 7 Agar

[所属分类:培养基配方] [发布时间:2021-12-20] [发布人:网站管理员2] [阅读次数:] [返回]

Tergitol 7 Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Lactose..............................................................20.0g

Agar ..................................................................13.0g

Peptone..............................................................10.0g

Yeast extract........................................................6.0g

Meat extract ........................................................5.0g

Tergitol-7.............................................................0.1g

Bromthymol Blue .............................................0.05g

TTC solution................................................... 5.0mL

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

TTC Solution:

Composition per 100.0mL:

Triphenyltetrazolium chloride ..........................0.05g

Preparation of TTC Solution: Add triphenyltet

razolium chloride to distilled/deionized water and

bring volume to 100.0mL. Mix thoroughly. Filter

sterilize.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to

995.0mL. Mix thoroughly. Gently heat and bring to

boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 50°C. Aseptically add 5.0mL of ster

ile TTC solution. Mix thoroughly. Pour into sterile

Petri dishes or distribute into sterile tubes.

Use: For the detection and enumeration of

coliforms. Lactose-fermenting bacteria appear as

yellow colonies. Lactose-nonfermenting bacteria ap

pear as blue colonies.