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Tergitol 7 Agar
[所属分类:培养基配方] [发布时间:2021-12-20] [发布人:网站管理员2] [阅读次数:] [返回]
Tergitol 7 Agar
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Agar ..................................................................15.0g
Lactose..............................................................10.0g
Yeast extract........................................................3.0g
Pancreatic digest of casein..................................2.5g
Peptic digest of animal tissue..............................2.5g
Tergitol 7.............................................................0.1g
Bromthymol Blue ..........................................25.0mg
TTC solution................................................... 3.0mL
pH 6.9 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
TTC Solution:
Composition per 100.0mL:
Triphenyl tetrazolium chloride ...........................1.0g
Preparation of TTC Solution: Add triphenyltet
razolium chloride to distilled/deionized water and
bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components to
distilled/deionized water and bring volume to
997.0mL. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Aseptically add 3.0mL of ster
ile TTC solution. Mix thoroughly. Pour into sterile
Petri dishes or distribute into sterile tubes.
Use: For the selective isolation and differentiation of
coliform bacteria based on lactose fermentation. Lactose
fermenting bacteria appear as yellow colonies. Lactose
nonfermenting bacteria appear as blue colonies.