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Tergitol 7 Broth

[所属分类:培养基配方] [发布时间:2021-12-20] [发布人:网站管理员2] [阅读次数:] [返回]

Tergitol 7 Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Lactose..............................................................10.0g

Yeast extract........................................................3.0g

Pancreatic digest of casein..................................2.5g

Peptic digest of animal tissue .............................2.5g

Tergitol 7.............................................................0.1g

Bromthymol Blue ..........................................25.0mg

TTC solution................................................... 3.0mL

pH 6.9 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

TTC Solution:

Composition per 100.0mL:

Triphenyl tetrazolium chloride ...........................1.0g

Preparation of TTC Solution: Add triphenyltet

razolium chloride to distilled/deionized water and

bring volume to 100.0mL. Mix thoroughly. Filter

sterilize.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to

997.0mL. Mix thoroughly. Gently heat while stirring

and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 25°C. Aseptically add

3.0mL of sterile TTC solution. Mix thoroughly.

Use: For the isolation and cultivation of coliforms,

Salmonella, and other enteric bacteria.