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Tetrathionate Broth (m-Tetrathionate Broth) (m-TT Broth)

[所属分类:培养基配方] [发布时间:2021-12-20] [发布人:网站管理员2] [阅读次数:] [返回]

Tetrathionate Broth

(m-Tetrathionate Broth)

(m-TT Broth)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Na2S2O3 ............................................................30.0g

CaCO3 ...............................................................10.0g

Pancreatic digest of casein..................................2.5g

Peptic digest of animal tissue..............................2.5g

Iodine-iodide solution ................................... 20.0mL

pH 8.0 ± 0.2 at 25°C

Iodine-Iodide Solution:

Composition per 20.0mL:

Iodine ..................................................................6.0g

KI ........................................................................5.0g

Preparation of Iodine-Iodide Solution: Add

iodine and KI to distilled/deionized water and bring

volume to 20.0mL. Mix thoroughly.

Preparation of Medium: Add components, ex

cept iodine-iodide solution, to distilled/deionized

water and bring volume to 980.0mL. Mix thoroughly.

Gently heat and bring to boiling. Do not autoclave.

Cool to 40°C. Add 20.0mL of iodine-iodide solution.

Mix thoroughly. Distribute into tubes in 10.0mL vol

umes. Use medium the same day it is prepared.

Use: For the selective isolation in the membrane fil

ter method of Salmonella species from foods.