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Tetrathionate Broth (m-Tetrathionate Broth)

[所属分类:培养基配方] [发布时间:2021-12-20] [发布人:网站管理员2] [阅读次数:] [返回]

Tetrathionate Broth

(m-Tetrathionate Broth)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Na2S2O3 ............................................................30.0g

Proteose peptone.................................................5.0g

Bile salts..............................................................1.0g

Iodine solution .............................................. 20.0mL

pH 8.0 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Iodine Solution:

Composition per 20.0mL:

Iodine ..................................................................6.0g

KI ........................................................................5.0g

Preparation of Iodine Solution: Add iodine and

KI to distilled/deionized water and bring volume to

20.0mL. Mix thoroughly.

Preparation of Medium: Add components, ex

cept iodine solution, to distilled/deionized water and

bring volume to 980.0mL. Mix thoroughly. Gently

heat and bring to boiling. Do not autoclave. Cool to

40°C. Add 20.0mL of iodine solution. Mix thorough

ly. Use medium the same day it is prepared.

Use: For the enrichment of Salmonella species in the

membrane filter method prior to placing the filter on

selective media such as Brilliant Green broth.