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Thiamine Assay Medium

[所属分类:培养基配方] [发布时间:2021-12-21] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................40.0g

Peptone .............................................................22.0g

Sodium acetate..................................................15.0g

Vitamin assay casamino acids ............................5.0g

K2HPO4...............................................................1.0g

KH2PO4...............................................................1.0g

MgSO4·7H2O......................................................0.4g

Adenine sulfate .................................................0.02g

FeSO4·7H2O......................................................0.02g

Guanine·HCl .....................................................0.02g

MnSO4·5H2O....................................................0.02g

NaCl..................................................................0.02g

Uracil ................................................................0.02g

L-Cystine ..........................................................0.2mg

p-Aminobenzoic acid.......................................0.2mg

Calcium pantothenate ......................................0.2mg

Niacin...............................................................0.2mg

Pyridoxine·HCl ................................................0.2mg

Riboflavin ........................................................0.2mg

Folic acid ..........................................................0.5μg

Biotin ................................................................0.8μg

pH 6.5 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Continue boiling for 2–3 min. Distribute into tubes or

flasks in 5.0mL volumes while swirling the flask to

disperse the precipitate. Add standard solutions or

test solutions and bring volume of each tube to

10.0mL with distilled/deionized water. Autoclave for

15 min at 15 psi pressure–121°C.

Use: For the microbiological assaying of thiamine

using Lactobacillus fermentum as the test organism.