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Thiamine Assay Medium LV

[所属分类:培养基配方] [发布时间:2021-12-21] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................20.0g

Pancreatic digest of casein................................20.0g

K2HPO4.............................................................10.0g

NaCl..................................................................10.0g

Sodium citrate...................................................10.0g

Yeast extract, thiamine-free ..............................10.0g

Sorbitan monooleate complex ............................2.0g

MgSO4·7H2O ......................................................1.6g

MnCl2·4H2O .....................................................0.28g

FeSO4·7H2O......................................................0.08g

pH 6.0 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Continue boiling for 2–3 min. Distribute into tubes or

flasks in 5.0mL volumes while swirling the flask to

disperse the precipitate. Add standard solutions or

test solutions and bring volume of each tube to

10.0mL with distilled/deionized water. Autoclave for

15 min at 15 psi pressure–121°C.

Use: For the microbiological assaying of thiamine

using Lactobacillus viridescens as the test organism.