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Thiogel® Medium

[所属分类:培养基配方] [发布时间:2021-12-21] [发布人:网站管理员2] [阅读次数:] [返回]

Thiogel® Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Gelatin...............................................................50.0g

Pancreatic digest of casein................................17.0g

Glucose ...............................................................6.0g

Papaic digest of soybean meal............................3.0g

NaCl....................................................................2.5g

Sodium thioglycolate ..........................................0.5g

Agar ....................................................................0.7g

Na2SO3................................................................0.1g

L-Cystine ...........................................................0.25g

pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water preheated to 50°C and bring

volume to 1.0L. Mix thoroughly. Let stand for 5 min.

Gently heat while stirring and bring to boiling. Dis

tribute into tubes, filling them half full. Autoclave for

15 min at 13 psi pressure–118°C. Pour into sterile

Petri dishes or leave in tubes.

Use: For the differentiation of microorganisms

based on their ability to liquefy gelatin.