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Thioglycolate Medium, Enriched (THIO Medium) (Thioglycolate Medium with Vitamin K1 and Hemin)

[所属分类:培养基配方] [发布时间:2021-12-21] [发布人:网站管理员2] [阅读次数:] [返回]

Thioglycolate Medium, Enriched

(THIO Medium)

(Thioglycolate Medium with

Vitamin K1 and Hemin)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Thioglycolate medium without indicator........... 1.0L

Hemin solution................................................ 0.5mL

Vitamin K1 solution ........................................ 0.1mL

pH 7.0 ± 0.2 at 25°C

Thioglycolate Medium without Indicator:

Composition per liter:

Pancreatic digest of casein................................17.0g

Glucose ...............................................................6.0g

Papaic digest of soybean meal............................3.0g

NaCl....................................................................2.5g

Agar ....................................................................0.7g

Sodium thioglycolate..........................................0.5g

L-Cystine ...........................................................0.25g

Na2SO3................................................................0.1g

Source: Thioglycolate medium without indicator is

available as a premixed powder from Oxoid and BD

Diagnostic Systems.

Preparation of Thioglycolate Medium with

out Indicator: Add components to distilled/deion

ized water and bring volume to 1.0L. Mix thoroughly.

Vitamin K1 Solution:

Composition per 100.0mL:

Vitamin K1 ..........................................................1.0g

Preparation of Vitamin K1 Solution: Add vita

min K1 to 99.0mL of absolute ethanol. Mix thorough

ly.

Hemin Solution:

Composition per 100.0mL:

Hemin .................................................................1.0g

NaOH (1N solution)...................................... 20.0mL

Preparation of Hemin Solution: Add hemin to

20.0mL of 1N NaOH solution. Mix thoroughly.

Bring volume to 100.0mL with distilled/deionized

water.

Preparation of Medium: Add 0.5mL of hemin

solution and 0.1mL of vitamin K1 solution to 1.0L of

thioglycolate medium without indicator. Mix thor

oughly. Distribute into screw-capped tubes or flasks.

Autoclave for 15 min at 15 psi pressure–121°C. Cool

tubes or flasks under 85% N2 + 10% H2 + 5% CO2.

Tighten caps.

Use: For the isolation, cultivation, and identification

of a wide variety of obligate anaerobic bacteria.

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Thioglycolate Medium, Enriched (THIO Medium) (Thioglycolate Medium with Vitamin K1 and Hemin)