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Thioglycolate Medium, Fluid (Fluid Thioglycolate Medium) (FTG) (BAM M146)

[所属分类:培养基配方] [发布时间:2021-12-21] [发布人:网站管理员2] [阅读次数:] [返回]

Thioglycolate Medium, Fluid

(Fluid Thioglycolate Medium)

(FTG)

(BAM M146)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein................................15.0g

Glucose ...............................................................5.0g

Yeast extract........................................................5.0g

NaCl....................................................................2.5g

Agar ..................................................................0.75g

L-cystine ..............................................................0.5g

Sodium thioglycolate ..........................................0.5g

Resazurin solution........................................... 1.0mL

pH 7.1 ± 0.2 at 25°C

Resazurin Solution:

Composition per 10.0mL:

Na-resazurin...................................................10.0mg

Preparation of Resazurin Solution: Add Na

resazurin to 10.0mL of distilled/deionized water. Mix

thoroughly. Prepare freshly.

Preparation of Medium: Add components, ex

cept sodium thioglycolate and resazurin solution, to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Add 0.5g sodium thioglycolate. Mix thoroughly. Ad

just pH to 7.1. Add 1.0mL resazurin solution. Mix

thoroughly. Distribute into tubes or flasks. Autoclave

for 20 min at 15 psi pressure–121°C.

Use: For the cultivation of both aerobic and anaero

bic organisms in the performance of sterility tests.