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Thioglycolate Medium without Glucose

[所属分类:培养基配方] [发布时间:2021-12-21] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein................................15.0g

Yeast extract........................................................5.0g

NaCl....................................................................2.5g

Agar ..................................................................0.75g

L-Cystine ...........................................................0.25g

Methylene Blue................................................2.0mg

Thioglycollic acid ........................................... 0.3mL

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. If medium becomes oxi

dized before use (Methylene Blue turns blue), heat in

a boiling water bath to expel absorbed O2. Cool to

25°C.

Use: For the cultivation of anaerobic, microaero

philic, and aerobic microorganisms. For use in steril

ity testing of a variety of specimens.