培养基配方

您现在的位置：网站首页 >> 培养基配方

Thioglycolate Medium without Glucose

[所属分类:培养基配方] [发布时间:2021-12-21] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein................................20.0g

NaCl....................................................................2.5g

K2HPO4...............................................................1.5g

Sodium thioglycolate ..........................................0.6g

Agar ....................................................................0.5g

L-Cystine .............................................................0.4g

Na2SO3................................................................0.2g

Methylene Blue................................................2.0mg

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat while stirring and bring

to boiling. Distribute into tubes or flasks, filling them

half full. Autoclave for 15 min at 15 psi pressure–

121°C.

Use: Use as a base for fermentation studies of anaer

obic bacteria and for the promotion of endospore for

mation.