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Todd-Hewitt Broth

[所属分类:培养基配方] [发布时间:2021-12-21] [发布人:网站管理员2] [阅读次数:] [返回]

Todd-Hewitt Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein................................20.0g

Infusion from 450.0g fat-free minced meat......10.0g

Glucose ...............................................................2.0g

NaHCO3 ..............................................................2.0g

NaCl....................................................................2.0g

Na2HPO4 .............................................................0.4g

pH 7.8 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Distribute into tubes or flasks. Auto

clave for 10 min at 10 psi pressure–115°C.

Use: For the cultivation of group A streptococci used

in serological typing, and for the cultivation of a va

riety of pathogenic microorganisms.