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Todd-Hewitt Broth (ATCC Medium 235)

[所属分类:培养基配方] [发布时间:2021-12-21] [发布人:网站管理员2] [阅读次数:] [返回]

Todd-Hewitt Broth

(ATCC Medium 235)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone .............................................................20.0g

Beef heart, solids from infusion..........................3.1g

Na2CO3 ...............................................................2.5g

Glucose ...............................................................2.0g

NaCl....................................................................2.0g

Na2HPO4.............................................................0.4g

pH 7.8 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of Group A streptococci

used in serological typing, and for the cultivation of

a variety of pathogenic microorganisms.