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Toluidine Blue DNA Agar (BAM M148)

[所属分类:培养基配方] [发布时间:2021-12-21] [发布人:网站管理员2] [阅读次数:] [返回]

Toluidine Blue DNA Agar

(BAM M148)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................10.0g

NaCl..................................................................10.0g

Tris(hydroxymethyl)aminomethane buffer.........6.1g

Deoxyribonucleic acid........................................0.3g

Toluidine Blue O.............................................0.083g

CaCl2, anhydrous.............................................1.1mg

pH 9.0 ± 0.2 at 25°C

Preparation of Medium: Add tris(hydroxymeth

yl)aminomethane buffer to distilled/deionized water

and bring volume to 1.0L. Mix thoroughly. Adjust

pH to 9.0. Add the remaining components, except

Toluidine Blue O. Mix thoroughly. Gently heat and

bring to boiling. Add Toluidine Blue O. Mix thor-

oughly. If used the same day, sterilization is not nec

essary. Cool to 50°C. Pour into sterile Petri dishes or

distribute into sterile tubes.

Use: For the cultivation and differentiation of Sta

phylococcus aureus from foods.