培养基配方

您现在的位置：网站首页 >> 培养基配方

Toluidine Blue DNA Agar

[所属分类:培养基配方] [发布时间:2021-12-21] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................10.0g

NaCl..................................................................10.0g

Tris(hydroxymethyl)aminomethane buffer.........6.1g

Deoxyribonucleic acid (DNA)............................0.3g

Toluidine Blue O.............................................0.083g

CaCl2, anhydrous .............................................1.1mg

pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components, ex

cept Toluidine Blue O, to distilled/deionized water

and bring volume to 1.0L. Mix thoroughly. Gently

heat and bring to boiling. Add Toluidine Blue O. Mix

thoroughly. Medium does not have to be sterilized if

used immediately. Pour into sterile Petri dishes or

distribute into sterile tubes. Allow tubes to cool in a

slanted position.

Use: For the cultivation and differentiation of bacte

ria based on their production of deoxyribonuclease

(DNase). Bacteria that produce DNase turn the medi

um pink.