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Tomato Juice Yeast Extract Milk Medium

[所属分类:培养基配方] [发布时间:2021-12-21] [发布人:网站管理员2] [阅读次数:] [返回]

Tomato Juice Yeast Extract

Milk Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Skim milk........................................................100.0g

Yeast extract........................................................5.0g

Tomato juice, filtered.................................. 100.0mL

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Filter canned tomato

juice through paper. Let stand overnight at 10°C. Add

remaining components and bring to 1.0L with dis

tilled/deionized water. Mix thoroughly. Distribute

into tubes or flasks. Autoclave for 15 min at 15 psi

pressure–121°C.

Use: For the cultivation and maintenance of a vari

ety of fastidious bacteria that require complex

growth factors, including Lactobacillus, Streptococ

cus, and Enterococcus species.