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TPEY Agar (Tellurite Polymyxin Egg Yolk Agar)

[所属分类:培养基配方] [发布时间:2021-12-22] [发布人:网站管理员2] [阅读次数:] [返回]

TPEY Agar

(Tellurite Polymyxin Egg Yolk Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..................................................................20.0g

Agar ..................................................................15.5g

Pancreatic digest of casein................................10.0g

Yeast extract........................................................5.0g

D-Mannitol ..........................................................5.0g

LiCl .....................................................................2.0g

Egg yolk emulsion (30% solution) ............. 100.0mL

Chapman tellurite solution............................ 10.0mL

Polymyxin B solution ..................................... 0.4mL

pH 7.1 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Egg Yolk Emulsion (30% Solution):

Composition per 100.0mL:

NaCl....................................................................0.6g

Egg yolk........................................................ 30.0mL

Preparation of Egg Yolk Emulsion (30% So

lution): Add NaCl and egg yolk to distilled/deion

ized water and bring volume to 100.0mL. Mix

thoroughly. Filter sterilize.

Chapman Tellurite Solution:

Composition per 100.0mL:

K2TeO3 ................................................................1.0g

Preparation of Chapman Tellurite Solution:

Add K2TeO3 to distilled/deionized water and bring

volume to 100.0mL. Mix thoroughly. Filter sterilize.

Polymyxin B Solution:

Composition per 100.0mL:

Polymyxin B .......................................................1.0g

Preparation of Polymyxin B Solution: Add

polymyxin B to distilled/deionized water and bring

volume to 100.0mL. Mix thoroughly. Filter sterilize.

Caution: Potassium tellurite is toxic.

Preparation of Medium: Add components—ex

cept 30% egg yolk emulsion, Chapman tellurite solu

tion, and polymyxin B solution—to distilled/

deionized water and bring volume to 890.0mL. Mix

thoroughly. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C. Aseptically add 100.0mL of sterile 30%

egg yolk emulsion, 10.0mL of sterile Chapman tellu

rite solution, and 0.4mL of sterile polymyxin B solu

tion. Mix thoroughly. Pour into sterile Petri dishes or

distribute into sterile tubes.

Use: For the recovery of staphylococci from foods

and other materials.

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TPEY Agar (Tellurite Polymyxin Egg Yolk Agar)