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Trypaflavin Nalidixic Acid Serum Agar (TNSA Agar)

[所属分类:培养基配方] [发布时间:2021-12-22] [发布人:网站管理员2] [阅读次数:] [返回]

Trypaflavin Nalidixic Acid Serum Agar

(TNSA Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Ionagar No. 2 ....................................................12.0g

Peptone..............................................................10.0g

Beef extract.........................................................3.0g

H2O ............................................................. 926.5mL

Bovine serum, heat inactivated..................... 50.0mL

Nalidixic acid solution.................................. 20.0mL

Trypaflavin solution........................................ 3.5mL

pH 7.2–7.4 at 25°C

Nalidixic Acid Solution:

Composition per 10.0mL:

Nalidixic acid....................................................0.02g

Preparation of Nalidixic Acid Solution: Add

nalidixic acid to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Trypaflavin Solution:

Composition per 10.0mL:

Trypaflavin..........................................................0.1g

Preparation of Trypaflavin Solution: Add try

paflavin to distilled/deionized water and bring vol

ume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components—ex

cept bovine serum, nalidixic acid solution, and try

paflavin solution—to distilled/deionized water and

bring volume to 926.5mL. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 45°–50°C. Aseptically

add 50.0mL of sterile bovine serum, 20.0mL of ster

ile nalidixic acid solution, and 3.5mL of trypaflavin

solution. Mix thoroughly. Pour into sterile Petri dish

es or distribute into sterile tubes.

Use: For the isolation and cultivation of Listeria spe

cies from preenriched specimens.