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Tryptic Nitrate Medium

[所属分类:培养基配方] [发布时间:2021-12-22] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Tryptose ............................................................20.0g

Na2HPO4.............................................................2.0g

Agar ....................................................................1.0g

Glucose ...............................................................1.0g

KNO3 ..................................................................1.0g

pH 7.6 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes in 10.0mL volumes. Autoclave

for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and differentiation of

Pseudomonas and related genera. For the differentia

tion of bacteria based on their reduction of nitrate to

nitrite. After incubation of the bacterium in tryptic ni

trate medium for 18–24 hr, sulfanillic acid and α-

naphthol reagents are added. Nitrate reduction is in

dicated by the development of a red to violet color.