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Trypticase Soy Polymyxin Broth (BAM M158)

[所属分类:培养基配方] [发布时间:2021-12-23] [发布人:网站管理员2] [阅读次数:] [返回]

Trypticase Soy Polymyxin Broth

(BAM M158)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1006.67mL:

Pancreatic digest of casein................................17.0g

NaCl....................................................................5.0g

Papaic digest of soybean meal............................3.0g

K2HPO4...............................................................2.5g

Glucose ...............................................................2.5g

Polymyxin B solution ................................... 6.67mL

pH 7.3 ± 0.2 at 25°C

Polymyxin B Solution:

Composition per 10.0mL:

Polymyxin B ...................................................0.015g

Preparation of Polymyxin B Solution: Add

polymyxin B to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept polymyxin B solution, to distilled/deionized wa

ter and bring volume to 1.0L. Mix thoroughly. Gently

heat and bring to boiling. Distribute into tubes in

15.0mL volumes. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C. Aseptically add

0.1mL of sterile polymyxin B solution to each tube.

Mix thoroughly.

Use: For the isolation and cultivation of Bacillus

cereus from foods.