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Trypticase Soy-Sheep Blood Agar (Tryptic Soy Blood Agar) (TSA Blood Agar) (BAM M159)

[所属分类:培养基配方] [发布时间:2021-12-23] [发布人:网站管理员2] [阅读次数:] [返回]

Trypticase Soy-Sheep Blood Agar

(Tryptic Soy Blood Agar)

(TSA Blood Agar)

(BAM M159)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1050mL:

Pancreatic digest of casein................................15.0g

Agar ..................................................................15.0g

Papaic digest of soybean meal............................5.0g

NaCl....................................................................5.0g

Sheep blood, defibrinated ............................. 50.0mL

pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components, ex

cept sheep blood, to distilled/deionized water and

bring volume to 1.0L. Mix thoroughly. Gently heat

and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C. Aseptically add

50.0mL sterile sheep blood. Mix thoroughly. Pour

into sterile Petri dishes in 20.0mL volumes or distrib

ute into sterile tubes.

Use: For the cultivation of a wide variety of fastidi

ous microorganisms. For the observation of hemolyt

ic reactions of a variety of bacteria. May be used to

perform the CAMP test for the presumptive identifi

cation of group B streptococci (Streptococcus aga

lactiae).