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TSC Agar, Fluorocult (Fluorocult TSC Agar) (Fluorocult Tryptose Sulfite Cycloserine Agar) (Tryptose Sulfite Cycloserine Agar, Fluorocult)

[所属分类:培养基配方] [发布时间:2021-12-24] [发布人:网站管理员2] [阅读次数:] [返回]

TSC Agar, Fluorocult

(Fluorocult TSC Agar)

(Fluorocult Tryptose Sulfite

Cycloserine Agar)

(Tryptose Sulfite Cycloserine

Agar, Fluorocult)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Tryptose ............................................................15.0g

Peptone from soymeal ........................................5.0g

Yeast extract........................................................5.0g

Na2S2O5 ..............................................................1.0g

Ammonium ferric citrate ....................................1.0g

D-Cycloserine......................................................0.2g

4-Methylumbelliferyl-phosphate

disodium salt ...........................................50.0mg

Source: This medium is available from Merck.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 45°–50°C. Pour into sterile Petri

dishes.

Use: For the isolation and enumeration of the vege

tative and spore forms of Clostridium perfringens in

foodstuffs. The culture medium complies with the

recommendations of the International Organization

for Standardization (ISO) (1978) and the DIN Norm

10165 for the examination of meat and meat prod

ucts. It also conforms with the APHA recommenda

tions for the examination of foods (1992). D-Cyclo

serine inhibits the accompanying bacterial flora and

causes the colonies which develop to remain smaller.

4-Methylumbelliferyl-phosphate (MUP) is a fluoro

genic substrate for the alkaline and acid phosphatase.

The acid phosphatase is a highly specific indicator

for C. perfringens. The acid phosphatase splits the

fluorogenic substrate MUP forming 4-methylumbel

liferone which can be identified by its fluorescence in

long wave UV light. Thus a strong suggestion for the

presence of C. perfringens can be obtained.