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培养基配方
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TSN Agar (Trypticase Sulfite Neomycin Agar)
[所属分类:培养基配方] [发布时间:2021-12-24] [发布人:网站管理员2] [阅读次数:] [返回]
TSN Agar
(Trypticase Sulfite Neomycin Agar)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Pancreatic digest of casein................................15.0g
Agar ..................................................................13.5g
Yeast extract......................................................10.0g
Na2SO3................................................................1.0g
Ferric citrate........................................................0.5g
Neomycin sulfate ..............................................0.05g
Polymyxin sulfate .............................................0.02g
Buffered thioglycolate solution..................... 50.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed
powder from BD Diagnostic Systems.
Buffered Thioglycolate Solution:
Composition per 50.0mL:
Buffer solution .............................................. 35.0mL
Sodium thioglycolate solution ...................... 15.0mL
Preparation of Buffered Thioglycolate Solu
tion: Combine components. Mix thoroughly. Auto
clave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C.
Buffer Solution:
Composition per 100.0mL:
Na2CO3 .............................................................28.0g
K2HPO4...............................................................5.7g
Preparation of Buffer Solution: Add compo
nents to distilled/deionized water and bring volume
to 100.0mL. Mix thoroughly.
Thioglycolate Solution:
Composition per 100.0mL:
Sodium thioglycolate........................................13.3g
Preparation of Thioglycolate Solution: Add
sodium thioglycolate to distilled/deionized water and
bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add components, ex
cept buffered thioglycolate solution, to distilled/
deionized water and bring volume to 950.0mL. Mix
thoroughly. Gently heat and bring to boiling. Auto
clave for 12 min at 13 psi pressure–118°C. Do not
overheat. Cool to 45°–50°C. Aseptically add buff
ered thioglycolate solution. Mix thoroughly. Pour
into sterile Petri dishes or distribute into sterile tubes.
Use: For the selective isolation of Clostridium per
fringens.