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TSYES Medium (Trypticase Soy Yeast Extract Starch Medium)

[所属分类:培养基配方] [发布时间:2021-12-24] [发布人:网站管理员2] [阅读次数:] [返回]

TSYES Medium

(Trypticase Soy Yeast

Extract Starch Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein................................17.0g

Agar ..................................................................15.0g

NaCl....................................................................5.0g

Papaic digest of soybean meal............................3.0g

K2HPO4...............................................................2.5g

Glucose ...............................................................2.5g

Yeast extract........................................................2.0g

Soluble starch......................................................1.0g

pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat while stirring and bring

to boiling. Distribute into tubes or flasks. Autoclave

for 15 min at 15 psi pressure–121°C. Pour into sterile

Petri dishes or leave in tubes.

Use: For the cultivation of Bacillus species.