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Tween 80 Hydrolysis Broth

[所属分类:培养基配方] [发布时间:2021-12-24] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 102.5mL:

NaHPO4 (0.066M solution) .......................... 61.1mL

KH2PO4 (0.066M solution)........................... 38.9mL

Neutral Red (0.1% solution)........................... 2.0mL

Tween 80......................................................... 0.5mL

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Combine components.

Mix thoroughly. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C.

Use: For the differentiation of Mycobacterium spe

cies. Strains that hydrolyze Tween 80 within 5 days

turn the medium pink to red.