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Tween 80 Hydrolysis Broth

[所属分类:培养基配方] [发布时间:2021-12-24] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 125.0mL:

Neutral Red.........................................................0.1g

Solution 1...................................................... 38.9mL

Solution 2...................................................... 61.1mL

Tween 80....................................................... 25.0mL

pH 7.0 ± 0.2 at 25°C

Solution 1:

Composition per 400.0mL:

KH2PO4.............................................................22.7g

Preparation of Solution 1: Add KH2PO4 to dis

tilled/deionized water and bring volume to 400.0mL.

Mix thoroughly.

Solution 2:

Composition per 400.0mL:

Na2HPO4 ...........................................................23.8g

Preparation of Solution 2: Add Na2HPO4 to dis

tilled/deionized water and bring volume to 400.0mL.

Mix thoroughly.

Preparation of Medium: Combine components.

Mix thoroughly. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C.

Use: For the differentiation of Mycobacterium spe

cies. Strains that hydrolyze Tween 80 within 5 days

turn the medium pink to red.