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Wagatsuma Agar (BAM M178)

[所属分类:培养基配方] [发布时间:2021-12-28] [发布人:网站管理员2] [阅读次数:] [返回]

Wagatsuma Agar

(BAM M178)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1050.0mL:

NaCl..................................................................70.0g

Agar ..................................................................15.0g

Mannitol............................................................10.0g

Peptone..............................................................10.0g

K2HPO4 ..............................................................5.0g

Yeast extract........................................................3.0g

Crystal Violet ...................................................1.0mg

Red blood cells.............................................. 50.0mL

pH 8.0 ± 0.2 at 25°C

Red Blood Cells:

Composition per 100.0mL:

Blood, human or rabbit............................... 100.0mL

Preparation of Red Blood Cells: Mix freshly

drawn human or rabbit blood with anticoagulant and

an equal volume of sterile 0.85% saline solution.

Centrifuge cells at 4000 × g at 4°C for 15 min. Pour

off saline and wash two more times with sterile sa

line. After last wash, pour off saline and resuspend

cells to their original volume.

Preparation of Medium: Add components, ex

cept blood, to distilled/deionized water and bring

volume to 1.0L. Mix thoroughly. Adjust pH to 8.0.

Place in a steam bath for 30 min. Do not autoclave.

Cool to 45°–50°C. Add 50.0mL of washed red blood

cells. Mix thoroughly. Pour into sterile Petri dishes.

Dry plates before using.

Use: For the cultivation and detection of thermo

stable hemolysin of Vibrio parahaemolyticus by the

Kanagawa reaction.