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WL Nutrient Broth (Wallerstein Laboratory Nutrient Broth)

[所属分类:培养基配方] [发布时间:2021-12-28] [发布人:网站管理员2] [阅读次数:] [返回]

WL Nutrient Broth

(Wallerstein Laboratory

Nutrient Broth)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................50.0g

Pancreatic digest of casein..................................5.0g

Yeast extract........................................................4.0g

FeCl3 ...................................................................2.5g

MnSO4·4H2O......................................................2.5g

KH2PO4.............................................................0.55g

KCl..................................................................0.425g

CaCl2·2H2O ....................................................0.125g

MgSO4·7H2O..................................................0.125g

Bromcresol Green...........................................0.022g

pH 5.5 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C.

Use: For the control of brewing and other fermenta

tion processes.