培养基配方

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Woods and Welton Agar

[所属分类:培养基配方] [发布时间:2021-12-28] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..................................................................23.4g

Casein hydrolysate............................................17.0g

Agar ..................................................................15.0g

Glycerol ............................................................10.0g

Pancreatic digest of gelatin.................................5.0g

Glucose ...............................................................5.0g

Papaic digest of soybean meal............................3.0g

Beef extract.........................................................3.0g

Yeast extract........................................................2.0g

Casamino acids, vitamin free..............................0.5g

Pancreatic digest of casein..................................0.5g

Na2SO3................................................................0.1g

pH 7.6 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Adjust pH to 7.6. Distribute into tubes or flasks. Au

toclave for 15 min at 15 psi pressure–121°C. Pour

into sterile Petri dishes or leave in tubes.

Use: For the cultivation of Vibrio alginolyticus.