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Wort Agar

[所属分类:培养基配方] [发布时间:2021-12-28] [发布人:网站管理员2] [阅读次数:] [返回]

Wort Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Malt extract.......................................................15.0g

Maltose............................................................12.75g

Dextrin ..............................................................2.75g

Glycerol ............................................................2.35g

K2HPO4...............................................................1.0g

NH4Cl .................................................................1.0g

Pancreatic digest of gelatin...............................0.78g

pH 4.8 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems and Oxoid.

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Boil for

1 min with mixing. Distribute into tubes or flasks. Au

toclave for 15 min at 15 psi pressure–121°C. Do not

overheat, as this will result in hydrolysis of the agar.

An additional 5.0g of agar can be used to make a firm

er agar. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and enumeration of yeasts.

The low pH of the agar selectively inhibits bacterial

growth.