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Wort Sucrose Agar

[所属分类:培养基配方] [发布时间:2021-12-28] [发布人:网站管理员2] [阅读次数:] [返回]

Wort Sucrose Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................25.0g

Yeast extract........................................................1.0g

Wort solution............................................... 500.0mL

Sucrose solution.......................................... 500.0mL

Wort Solution:

Composition per 500.0mL:

Malt extract.......................................................55.0g

Preparation of Wort Solution: Add malt extract

to distilled/deionized water and bring volume to

500.0mL. Mix thoroughly.

Sucrose Solution:

Composition per 500.0mL:

Sucrose..............................................................50.0g

Preparation of Sucrose Solution: Add sucrose

to distilled/deionized water and bring volume to

500.0mL. Mix thoroughly.

Preparation of Medium: Combine components.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Pour into sterile Petri dish

es or distribute into sterile tubes.

Use: For the cultivation and maintenance of As

pergillus oryzae.