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XL Agar Base (Xylose Lysine Agar Base)

[所属分类:培养基配方] [发布时间:2021-12-28] [发布人:网站管理员2] [阅读次数:] [返回]

XL Agar Base

(Xylose Lysine Agar Base)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................13.5g

Lactose................................................................7.5g

Sucrose................................................................7.5g

L-Lysine...............................................................5.0g

NaCl....................................................................5.0g

Xylose .................................................................3.5g

Yeast extract........................................................3.0g

Phenol Red........................................................0.08g

Thiosulfate-citrate solution........................... 20.0mL

pH 7.5 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Thiosulfate-Citrate Solution:

Composition per 100.0mL:

Na2S2O3 ............................................................34.0g

Ferric ammonium citrate.....................................4.0g

Preparation of Thiosulfate-Citrate Solution:

Add components to distilled/deionized water and

bring volume to 100.0mL. Mix thoroughly.

Preparation of Medium: Add components, ex

cept thiosulfate-citrate solution, to distilled/deion

ized water and bring volume to 980.0mL. Mix

thoroughly. Gently heat while stirring and bring to

boiling. Distribute into tubes or flasks. Autoclave for

10 min at 14 psi pressure–118°C. Cool to 55°C.

Aseptically add 20.0mL of the sterile thiosulfate-cit

rate solution. Mix thoroughly. Pour into sterile Petri

dishes or leave in tubes.

Use: For the isolation, cultivation, and differentia

tion of enteric pathogens. Nonfermenting xylose/lac

tose/sucrose bacteria appear as red colonies. Xylose

fermenting, lysine-decarboxylating bacteria appear

as red colonies. Xylose-fermenting, lysine-nondecar

boxylating bacteria appear as opaque yellow colo

nies. Lactose or sucrose-fermenting bacteria appear

as yellow colonies.