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Yeast Nitrogen Base

[所属分类:培养基配方] [发布时间:2021-12-29] [发布人:网站管理员2] [阅读次数:] [返回]

Yeast Nitrogen Base

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

(NH4)2SO4...........................................................5.0g

KH2PO4...............................................................1.0g

MgSO4·7H2O ......................................................0.5g

NaCl....................................................................0.1g

CaCl2·2H2O.........................................................0.1g

DL-Methionine...................................................0.02g

DL-Tryptophan...................................................0.02g

L-Histidine·HCl .................................................0.01g

Inositol .............................................................2.0mg

KI .....................................................................1.0mg

H3BO3 ..............................................................0.5mg

ZnSO4·7H2O ....................................................0.4mg

MnSO4·4H2O ...................................................0.4mg

Thiamine·HCl ..................................................0.4mg

Pyroxidine·HCl ................................................0.4mg

Niacin...............................................................0.4mg

Calcium pantothenate ......................................0.4mg

p-Aminobenzoic acid.......................................0.2mg

Riboflavin ........................................................0.2mg

FeCl3 ................................................................0.2mg

Na2MoO4·4H2O ...............................................0.2mg

CuSO4·5H2O..................................................0.04mg

Folic acid ..........................................................2.0μg

Biotin ................................................................2.0μg

pH 5.5 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C. Alternate

ly for carbon assimilation tests, prepare a 10×

concentrated solution by adding components to dis

tilled/deionized water and bring volume to 100.0mL.

Mix thoroughly. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C. Prepare a

carbohydrate solution by adding 0.5g of carbohy

drate to 90.0mL of distilled/deionized water. Mix

thoroughly. Filter sterilize. Aseptically add 0.5mL of

the 10× concentrated solution to 4.5mL of the filter

sterilized carbohydrate solution. Mix thoroughly.

Use: For carbohydrate assimilation tests in the charac

terization and identification of yeasts.

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Yeast Nitrogen Base