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Yeast Synthetic Medium with 5′-dTMP

[所属分类:培养基配方] [发布时间:2021-12-29] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................20.0g

Agar ..................................................................15.0g

Yeast nitrogen base without amino acids............6.7g

Casamino acids without vitamins .......................2.0g

2′-Deoxythymidine-5′-monophosphate ..........7.2mg

Preparation of Medium: Add glucose, yeast ni

trogen base without amino acids, casamino acids

without vitamins, and 2′-deoxythymidine-5′-mono

phosphate to distilled/deionized water and bring vol

ume to 200.0mL. Mix thoroughly. Filter sterilize.

Add agar to distilled/deionized water and bring vol

ume to 800.0mL. Mix thoroughly. Gently heat and

bring to boiling. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 50°C. Aseptically combine the

two sterile solutions. Mix thoroughly. Pour into ster

ile Petri dishes or aseptically distribute into sterile

tubes.

Use: For the cultivation and maintenance of Saccharo

myces cerevisiae.