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Yeast Synthetic Minimal Medium

[所属分类:培养基配方] [发布时间:2021-12-29] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

D-Glucose..........................................................20.0g

Agar ..................................................................15.0g

(NH4)2SO4...........................................................5.0g

KH2PO4...............................................................1.0g

MgSO4·7H2O ......................................................0.5g

NaCl....................................................................0.1g

CaCl2·2H2O.........................................................0.1g

Inositol .............................................................2.0mg

H3BO3 ..............................................................0.5mg

ZnSO4·7H2O ....................................................0.4mg

MnSO4·4H2O ...................................................0.4mg

Thiamine·HCl ..................................................0.4mg

Pyridoxine·HCl ................................................0.4mg

Niacin...............................................................0.4mg

Calcium pantothenate ......................................0.4mg

p-Aminobenzoic acid.......................................0.2mg

Riboflavin ........................................................0.2mg

FeCl3 ................................................................0.2mg

Na2MoO4·4H2O ...............................................0.2mg

KI .....................................................................0.1mg

CuSO4·5H2O ..................................................0.04mg

Folic acid.......................................................... 2.0μg

Biotin ............................................................... 2.0μg

pH 5.6 ± 0.2 at 25°C

Preparation of Medium: Add agar to 900.0mL

of distilled/deionized water. Mix thoroughly. Gently

heat and bring to boiling. Distribute into tubes or

flasks. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C. In a separate flask, add re

maining components to 100.0mL of distilled/deion

ized water. Mix thoroughly. Filter sterilize.

Aseptically combine the two sterile solutions. Mix

thoroughly. Pour into sterile Petri dishes.

Use: For the cultivation of a wide variety of het

erotrophic microorganisms.