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Yeast Synthetic Minimal Medium

[所属分类:培养基配方] [发布时间:2021-12-29] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................20.0g

Agar ..................................................................15.0g

Yeast nitrogen base without amino acids............6.7g

Preparation of Medium: Add glucose and yeast

nitrogen base without amino acids to distilled/deion

ized water and bring volume to 100.0mL. Mix thor

oughly. Filter sterilize. Add agar to distilled/

deionized water and bring volume to 900.0mL. Mix

thoroughly. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

50°C. Aseptically combine the two sterile solutions.

Mix thoroughly. Pour into sterile Petri dishes or asep

tically distribute into sterile tubes.

Use: For the cultivation and maintenance of Pichia an

gusta and Saccharomyces cerevisiae.